Role of Endogenous H2S in the Accumulation of Extracellular Matrix of the Diabetic Nephropathy

Abstract

Abstract: Objective To explore changes of fibronectin(FN) in the kidney tissues of diabetic nephropathy)(DN) rats, and to study the role of endogenous H₂S in the pathological development of diabetic nephropathy. Methods Twenty-eight rats(male, Sprague-Dawley) were randomly divided into four groups: control group, diabetic nephropathy group, DN+NaHS(H₂S donor) group and DN+DL-propargylglycine(PAG, H₂S synthetase inhibitor) group. Relative location and expression of FN in kidney tissues were measured respectively by Western blotting and immunohistochemistry. Results FN was positive in both the intraglomerular mesangial region and the extracellular matrix(EMC) in kidney tissues of DN group, and its expression level increased significantly. Western blotting: DN group (48 896±779) vs control group(38 531±902), P<0.01. Compared with that of DN group and DN+PAG group, NaHS significantly inhibited the over-expression of FN, Western blotting: DN+NaHS group(42 737±1 196) vs DN group(48 896±779) and DN+PAG group(48 252±1 068), P<0.01. Conclusion Endogenous H₂S can alleviate the pathological progression of DN by inhibiting the accumulation of ECM.

Key words: hydrogen sulfide, diabetic nephropathy, fibronectin, Western blotting

CLC Number:

R 587.2

HUANG Wen;LI Xiao-lu;CHEN Shen;ZHANG Hong;YANG Min. Role of Endogenous H₂S in the Accumulation of Extracellular Matrix of the Diabetic Nephropathy[J]. Journal of Capital Medical University, 2009, 30(2): 133-136.

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Role of Endogenous H₂S in the Accumulation of Extracellular Matrix of the Diabetic Nephropathy

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