Table of Content

15 January 1998, Volume 19 Issue 1

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论著

The Study of Hemiparkinsonism in Primate after Unilateral Internal Carotid Artery Infusion of MPTP

Zhang Jinlu;Liu Yujun;Lu Qiang;Wang Yuanshen;Xu Qunyuan;Li Kuncheng;Gu Yebing

1998, 19(1):  1-4. 

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In order to make a primate model for Parkinsonizm,13 monkeys(Macaque)were ipsilaterally injected through the common carotid artery by 1-methyl-4-pheny-1,2,3,6-tetrahydropyridine(MPTP). Among them, the extrenal carotid artery in 6 monkeys was clipped before the injection. All animals with symptoms showed to have a decrease of density in the area of the substantia nigra on the image of MRI after 1 month of injection. In addition, the tyrosine hydroxylase immunoreactive neurons in the ventral midbrain area on the injection side were decreased in these monkeys. The results showed that ipsilateral injection of MPTP into the common carotid artery with a temporary blockage of the external carotid artery was a good technique for producing the primate model for Parkinsonizm.

Morphologic Changes of Rat Model of Moentjang Tina

Wang Hongchao;Chen Ruifen;Song Aili;Liu Guozhen;Han Zhengzheng;Yao Yaling

1998, 19(1):  5-8. 

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Rat model of Moentjang tina was produced by imitating Moentjang tina of human beings with different dose of Tung oil(Aleuriter fordii), which had made 55 people poisoned. The result showed that different pathological changes, such as interstitial edema of lung, hydropic degeneration of proximal tubule, fatty degeneration of hepatocyte and loose cytoplasm of myocardium, happened in groups of different dose of Tung oil, and that the severity of injury paralleled somewhat to the amount of oil used. The experiment indicates that Tung oil can cause comprehensive injuries of different organs.

The Effect of Gangliosides and Animal Protecting Effect in Acute and Repetitive Hypoxia

Pan Ying;Yang Dian'er;Yu Peilan

1998, 19(1):  9-12. 

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Repetitive hypoxia method was used to study the adaptation of mice to intermittent hypoxia and the effect of gangliosides. The activity of GSHpx was decreased(P<0.01), not only in blood but also in brain. Under acute repetitive hypoxia for 4 times, the activity of GSHpx in brain was increased(P<0.05)as compared with that under the hypoxia for one time. At that time, the gangliosides in the membrane of brain cell were decreased(P<0.01). It showed that Gls and the activity of GSHpx were interrelated. The contents of norepinephrine in brain were lower than the controls(P<0.01). The results indicated that the system of β receptor adenyl cyclase was not injured by acute and repetitive hypoxia.

A Long Term Toxicity Test of Mycobacterial Polysaccharides

Wu Ping;Su Yilan;Chen Zhenliang;Liu Zhen;Zhang Jie;Li Juan;Jiang Zhicheng;Zhao Wenming;Chen Ren

1998, 19(1):  13-18. 

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long term toxicity test of Mycobacterial polysaccharides(MPS)was carried out. Experimental groups with three different doses(1.5、7.5 and 15.0 μg/kg)of MPS were compared with the control in the parameters of growth ratio, coefficieny of food utilization, routine and specific tests of blood, OW/BW ratio and pathological examinations. Results showed no significant statistical differences between the experimental animals and controls. This study shows that MPS with such test doses has no adverse effect to the rats denoting that MPS can be used for long term treatment.

Determination and Effects of the Cholesterol Biosynthetic Inhibitor Produced by Monascus

Yu Jin;Zhu Changren

1998, 19(1):  19-22. 

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Atherosclerotic rabbits were treated by administrating Monascus. Changes of atherosclerosis and hyperlipidemia were observed. The experiments confirmed that Monascus may reduce serum cholesterol concentration in hypercholesterolemic rabbits and cease from progress of atherosclerosis. The metabolite of Monascus was determined by using the thin layer chromatographic qualitative analysis method and the HPLC quantitative analysis method. The results showed that lovastatin, an inhibitor of biosythesis of cholesterol, was produced by Monascus.

Calcineurin Activities in Human Fetal Brains of Different Developmental Periods Compared with Those in Brain from Alzheimer Disease

Zhou Chunlai;Xue Qiming;Wei Qun;Li Hong

1998, 19(1):  23-26. 

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Microtubule associated protein tau from Alzheimer disease(PHF-tau)and fetal brains(F-tau)are known to be hyperphosphorylated, though PHF-tau usually has been considered as an abnormally phosphorylated form of tau, calcineurin(CaN)is the only Ca²⁺/CaM dependent phosphatase for tau, which is enriched in human brain, might dephosphorylate PHF tau and F tau in vitro. In the present study, P nitrophenyl phosphate was used as substrate to measure CaN activities in 15 fresh fetal and infant brains, 2 brains from adult and one from Alzheimer disease. All these samples were dissected from 5 regions, i. e. frontal, hippocampal, occipital, basal ganglia and cerebellum. The results showed that the topographical difference of CaN might correlate with their physiological functions. The specific activity of CaN in one week newborn infan't brain appeared to have a higher level which was consistent with the time interval that F-tau was dephosphorylated. In the Alzheimer's brain, the specific activity of CaN decreased approximately to the respective level of those fetal brains of 17～20 weeks, suggesting that the formation of PHF tau might result from the decreasing CaN activity. Also, the hyperphosphorylation of the neuronal proteins might be the result of augmented activity of protein kinase, of reduced activity of CaN and other phosphatase, or both.

Morbidity and Prognosis Constitute Ratio of 2 792 Patients with Acute Myocardial Infarction During 20 Years Periods

Luo Zhuwei;Xu Zhimin;Cui Liang;Hu Dayi

1998, 19(1):  27-30. 

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The 20 years data from 2 792 cases of acute myocardial infarction(AMI)were divided into 4 groups in every five years, and the morbidity, mortality and prognosis were compared. The results showed, during the last 15 years, the total case numbers admitted to hospital were increased gradually for every five-year period. During the recent 10 years, the aged cases increased significantly, and the case number complicated with heart failure rose up and that with arrhythmias went down obviously. For constitute ratio of death, there was a trend that aged cases and reinfarction increased steadily. Most deaths resulted from heart failure,ventricular fibrillation or cardiac shock, the main death cause was heart failure especially for aged AMI. Therefor, It is the key measure for AMI cases to be hospitalized to prevent from heart failure.

Polymerase Chain Reaction for the Diagnosis of Pulmonary Tuberculousis

Liu Shuang;Yao Tianqiao;Xu Li;Qi Yahui;Yin Hong;Yao Qingying;He Cuizhen;Gao Tianxiang

1998, 19(1):  31-33. 

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Polymerase chain reaction(PCR)was applied to detect DNA of mycobacterium tuberculosis(TB)in 78 clinical samples. Methods: For each sample, TB-PCR, the positive rate of acid fast staining, tuberculin skin test and tuberculous antibody were made. The results of PCR were compared with those of conventional procedures and clinical diagnosis standard. Results: It was found that the positive rate of acid-fast staining, tuberculin skin test, tuberculous antibody and PCR were 3.85%、17.95%,11.54% and 46.15% respectively in 78 tuberculous samples. The sensitivity of PCR was much higher than the former diagnostic procedures. Conclusions: up to now, the results showed that the PCR was a valuable detective tool for diagnosis and differential diagnosis of tuberculosis. Diagnosis of tuberculousis still need to be determined by the patient's chlinical menfestions and others methods.

Endocrine Abnormality in Simple Obese Children

Hua Qi;Wang Yuqin;Wang Lihong;Wang Yongjun

1998, 19(1):  34-36. 

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The relationship between endocrine hormone and obesity was studied in 75 simple obese children of ages 6～15 and 59 age matched healthy children. A significant increase in insulin and E2 and decrease in human growth hormone and prolactin in the obese than the normal controls were observed(P all<0.01). There were no differences between two groups in triiodothyronine and COR. Serum insulin level was correlated positively to body mass index(r=0.61,P<0.001), age(r=0.40,P<0.01),height(r=0.50,P<0.001)and weight(r=0.62,P<0.001). Stepwise multivariate regression analysis disclosed that body mass index alone and jointly correlatedwith insulin level.

Correlation between Behavior and Endocrine Changes in Children with Simple Obesity

Liu Chunling;Wang Yuqin;Wang Lihong;Hu Liangping;Shang Jianwen

1998, 19(1):  37-40. 

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The aim of present study is to investigate the mechanism of simple obesity in childhood from the neuropsychology and neuroendocrinology points of view. The child behavior checklist(CBCL)and plasma concentrations of several hormones were measured in 65 children with simple obesity, whose body weight was more than 20% heavier than standard weight. 55 healthy children were taken as normal control in the study. The results showed that obese children had behavior deficit manifested by lower ability of social communication(P<0.05)and difficulty of behavioral problem(P<0.001). Abnormality of endocrine metabolism was found in obese children, showing an increase of plasma level of insulin and a decrease of growth hormone and prolactin. Their behavioral deficit and endocrine changes were related to sex and the degree of obesity. The behavioral problem in obese children was correlated to their endocrine metabolism with r=0.477(P<0.05). The correlation mainly existed between social communication and epinephrine or prolactin. The results demonstrate that children with simple obesity have, to various extent, neuropsychological deficit and abnormal endocrine metabolism.

Association between ACE Gene Polymorphism and Diabetic Retinopathy

Hu Wei;Wang Li;Liu Chunxia

1998, 19(1):  41-44. 

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o clarify whether angiotensin converting enzyme(ACE)insertion(I)/deletion(D)gene polymorphism attributes to the development of non-insulin dependent diabetes mellitus(NIDDM)and diabetic retinopathy. A fragment of 287 bp Alu sequence in inron 16 of ACE gene was used as I/D polymorphic marker. After PCR(polymerase chain reaction)amplication of DNA fragment, 1% agarose gel electrophoresis was undertaken to analyse the PCR products. Results: There was no significant difference in the frequency of ACE genotypes between 87 NIDDM patients and 50 normal subjects. There was no significant difference in the insertion(I)and deletion(D)alleles or frequencies of DD, DI, or II genotypes between two subgroups of NIDDM patients with and without diabetic retinopathy respectively(P<0.02). These results suggested that there was no association between ACE gene polymorphism and diabetic retinopathy.

The Sarcoidosis of Nervous System

Yu Sen;Chen Bingliang;Wang Jiarui;Wei Gangzhi;Zhang Jinlan

1998, 19(1):  45-48. 

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In order to improve the understanding of the nervous system damages affected by sarcoisis this paper reported 92 patients suffered from sarcoidosis with the damages of nervous system. In one of the 92 cases brain sarcoidosis was affirmed by autopsy.Damages of meninges, cranial nerve, spinal nerve and peripheral nerve were presented in four cases. Symptoms included the clinical manifestations of epilepsy, intracranial hypertension, motor weakness, facial paralysis, and dysopia. Sarcoidosis can bring about manifold clinical manifestations.In cases of brain, spinal cord, cranial or pheripheral nerve damages induced symptoms with an obscure origin, sarcoidosis should be suspected of.

Contrast between Simvastatin and Lovastatin in Treatment of Patients with Hyperlipoproteins

Gao Guojing;Hua Qi

1998, 19(1):  49-50. 

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Forty patients with hypercholesterolemia were divided into two subgroups, twenty of them were treated with simvastatin 5 mg qn, and twenty of them were treated with lovastatin 20 mg qn. The results showed that after complete 8～12 weeks of therapy, total cholesterol and low density lipoprotein decreased and high density lipoprotein increased in both grorps, simvastatin was more efficacious than lovastatin.

Measurement of Serum Alphafetoprotein in Patients with Primary Hepatocellular Carcinoma and Chronic Active Hepatitis by Immunoassay System Imx

Kong Shuling;Min Fuyuan;Wang Shaoxiang

1998, 19(1):  51-53. 

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Using fully automated immunoassay system Imx, the serum alpha fetoprotein(AFP)levels in patients with primary hepatocellular carcinoma(PHC)and chronic active hepatitis(CAH)were quantitatively determined. Results: In 75% of patients with PHC, the serum AFP levels were unnormal. In 11 patients, before and after operation, the serum AFP levels were observed dynamically. The serum AFP levels were elevated in 21.8% of 110 patients with CAH. In 13 of 15 cases, the serum AFP levels were from 14.27 nmol/L to normal. In another 2 cases, the serum AFP levels were persistently high, denoting a poor prognoses. Changes in contents of AFP coincided with changes of disease course and prognosis of PHC and CAH.

Assessment of Nutritional Status and Determination of Free Amino Acid in Patients with Post Hepatitis Cirrhosis Ascites

Yu Shuqing;Guo Yanbin;Meng Daixian

1998, 19(1):  54-56. 

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The nutritional status and plasma free amino acid(PFAA)of 18 patients with post hepatitis cirrhosis ascites(HLCA)were assessed before and after the treatment with medication and diet. The result showed that triceps skinfold fat(TSF),mid arm muscle circumference(MAMC),the level of some essential amino acids(EAA)and conditioned essential amino acids(CEAA)were singificantaly increased after the treatment(P<0.01,P<0.05).There was a good correlation between the ratio of branched chain amino acid(BCAA)to aromatic amino acid(AAA)and TSF. It is suggested that ascites may worsen the nutritional status in post hepatitis cirrhosis and the complex treatment with diet is extremely important. It is confirmed that the level of PFAA can reflect the nutritional status of the patients.

A Study on the Cervical Erosion and Chlamydia Trachomatis Infection

Lin Qing;Zhang Yueqing;Zhou Mo

1998, 19(1):  57-60. 

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To study the cervical Ct infection and treatment among the cervical erosion patients cervical Ct was examined from 119 case of cervical erosion patients and 52 cases of non cervical erosion patients with PCR technique. The cervical Ct positive patients were treated with Minocin and Albothyl respectively. Results: The Ct positive rate was 32.8% in the cervical erosion patients group and 7.5%(P<0.01)in the non erosion group. The cervical erosion patients with symptom or with complicated pelvic inflammatory disease showed a high rate of Ct positive. After a course of Minocin and Albothyl treatment the effective rates were 100% and 93.8% respectively for the cervical Ct infection, and 71.4% and 81.3% respectively for the cervical erosion. Conclusions: The Ct infection rate rose up in the cervical erosion patients and the Ct infection might be one of the reasons causing pelvic inflammatory disease. Minocin and Albothyl are both effective for curing Ct infection and cervical erosion.