Table of Content

15 October 1996, Volume 17 Issue 4

Previous Issue    Next Issue

论著

Effect of Different Degree of Hypoxia on Adaptation to Acute Repetitive Hypoxia

Li Haitao;Lu Guowei

1996, 17(4):  247-251. 

Asbtract ( 541 )   PDF (2506KB) ( 260 )  

Related Articles | Metrics

Experiments were conducted on SD rats.Three different limrts of tolerance, point of peak breath failing (group A) ,appearance of first gasping (group B) and last breath (group C) were used as the start of next exposure,respectively.As exposure went on,anmals' state and cortical electrical activity increased in group A while deceased in group B and C.The to lerance time of the second ,third and fourth run was 1.03, 1.03, 1.05 times in group A; 1.20 ,1.50 and 2.16 times in group B;and 1.43, 1.91 and 2.74 times in group C,respectively longer than that of their own first run.These results indicate that adaptation to hypoxia is related to inhibition of the central nervous system and the extent of increase in tolerance seems to be correlated to the extent of inhibition.

F-actin Microfilaments in the Outer Cells of Multicellular Tumor Spheroid (HEP 2)with Different Culture Duration

Li Wehong;Gao Minggeng;Ma Yanhong

1996, 17(4):  252-254. 

Asbtract ( 507 )   PDF (481KB) ( 172 )  

Related Articles | Metrics

We have compared the cytoskeleton of F-actin microfilaments in the outer cells of multicellular tumor spheroids(HEp 2) with different culture duration by staining with TRITC labeled phalloidin.Results showed that many F-actin aggregates appeared in the outer cells with the prolongation of MTS culture.These aggregates are dot-like with diameter 0.3～1.0 μm.The reorganization of F-actin microfilaments may not only be related to the greater potential capacity of outer cells to invade and metastasis ,but may also reflect the physical condition of the outer cells.

论著

Difluoromethyl Ornithine Reduced Delayed Neuronal Death in the Rat Hippocampus Following Transient Brain Ischemia

Hu Ying'an;Zhang Maoxian

1996, 17(4):  255-258. 

Asbtract ( 543 )   PDF (316KB) ( 202 )  

Related Articles | Metrics

Delayed neuronal death occured in the rat hippocampus following brief ischemia.We study and evaluate the effects of difluoromethyl ornithine (DFMO).ODC inhibitor, on the survival of neurons in the hippocampal CA₁ subfield.The pyramidal cell density in the CA₁ region is used as index of the effects.The results of experiment showed that DFMO has considerably reduced neuron loss in the CA₁ region.The favorabal effects are obviously dose-dependent and administration time-dependent.Furthermore, the results suggest that polyamine and ODC might play an importent role in the genesis of the delayed neuronal death following transient cerebral ischemia.

Comparative Study on Body Weight and Fat-reducing Effects of Double-reducing Decoction (Shuang Jiang Tang )and Fenfluramine in Rat

Liu Sumei;Xie Yan;Wu Xuebin;Zhang Maoxian;Li Xiaoyi;Wang Xinrong;Wei Kaiyuan

1996, 17(4):  259-262. 

Asbtract ( 478 )   PDF (2492KB) ( 195 )  

Related Articles | Metrics

The body weight and fat-reducing effects of double-reducing decoction and fenfluramine was investigated.The results indicated that double-reducing decoction is good for body weight and fat reducing in rat.It can lower body weight after two days of treatment.This effect continued for 90 days until the experiment was over.It can also lower the levels of serum cholesterol, triglycericles and β-lipoprotein.Its effects are better than that of fenfluramine.Double-reducing decoction is high effective, nontoxic and is better than fenfluramine.

Comparison of Mutagenicity of Airborne Particulates for Strain TA98 with that for its New Derivative Strains

Kong Xianhuan;Yin Xuejue;Wang Hong;Xiao Zhongxin;Chu Jinhua;Li Xianghong

1996, 17(4):  263-266. 

Asbtract ( 604 )   PDF (2368KB) ( 168 )  

Related Articles | Metrics

The mutagenicity of airborne particulates from differential function districts in Shenyang was studied with salmonella typhimurium strain TA98, the classically beliered sensitive strain, and its new derivative strains TA98NR, TA98/1 ,8DNP6, YG 1021 and YG1024,and sensitivity of these new strains were compared with that of TA98.The results indicated that the mutagenicity of samples for YG1024,YG1021 and TA98 were significantly stronger than that for TA98NR and TA98/1, 8DNP₆.Strain YG1024 was more specific to the mutagenicity of samples than TA98.This strongly suggests the presence of nitrated polycyclic aromatic hydro-carbons (NO₂-PAHs)in these particulates and that nitropyrenes must be the major direct-acting mutagens, especically dinitropyrenes (DNPs) .YG1024 can become the ralatively sensitive indicating strain in detecting mutagenicity of samples of environment.The results indicated samples from industrial and residential districts contained more NO₂-PAHs than that from other districts.

The Study of the Relationship between Laminin and Intracranial Astrocytoma Differentiation and Metastasis

Shi Xiaolin;Weng Jing;Xu Qing;Liang Yuanjing;Xu Qingzhong

1996, 17(4):  267-269. 

Asbtract ( 603 )   PDF (504KB) ( 184 )  

Related Articles | Metrics

The distribution of laminin (LN) in astrocytoma of various stages of differentiation were observed to study the relationship between laminin and intracranial astrocytoma differentiation and metastasis.LSAB immunohistochemistry method was showed laminin.The laminin distributed mainly in the glial and vascular basement membrane.Astrocytoma show structure of ring upon ring around blood vessels.Glioblastoma show multilayer fibriosis structure.The LN reaction of the vascular basement membrane dicreased,showed positive.According to the malignancy of the tumor.

论著

The Discuss about Nurition of Long Life People and Factors of Long Life

Meng Daixian;Jiang Baoji

1996, 17(4):  270-273. 

Asbtract ( 647 )   PDF (423KB) ( 303 )  

Related Articles | Metrics

long life people who were all over 90 years old in Beijing, Niu Street area were investigated.Some physical examinaltions and investigations of the status in diet and the habit of diet and life have been done.The results show that the incidence of long life people in Huinationality is 2.25 times than that of Han(P<0.01).The incidence of long life in women is 2.33 times than that of man (P<0.01).Body Mass Index (BMI) of men and women were 20.80 and 19.54 respectively.Most of them were short and slight in figure,none was fat.Their intakes of energy、protein and fat as well as carbohydrate were all in the low side.The habit on diet were reasonable and light.Most of them were neither drinking nor smoking while intaking more tea and less salt.

Plasma Endothelin Dynamic Change and Clinical Significance in AMI, Angina Pectoris and Heart Failure

Bian Yantao;Qin Mingzhao;Xu Yushen;Liu Yuantao;Li Qinglang

1996, 17(4):  274-276. 

Asbtract ( 716 )   PDF (2248KB) ( 157 )  

Related Articles | Metrics

Plasma endothelin (ET) with radioimmunoassay was messured respectively in AMI group (23 patients) angina pectoris group (26 patients) and coronary heart disease heart failure group(9 patients).ET in three groups was much higher than in control group.ET became normal one week after attack in AMI group.ET was highest in angina group at angina onset and returned to normal in one week as the symptoms relieved.ET was relevant to the degree of heart failure(HF).It was higher in Ⅳ grade HF than in Ⅲ grade HF and reduced with remission at a slower rate.ET was obvious higher in AMI group than in angina group.We consider that ET is correlated with myocardial ischemia , anoxia and cardiac function status.So ET can be used as the parameter of judging cardiac function status and assistant parameter of differiential diagnosis between AMI and angina.ET also can be used in dynamic observating on patients of post AMI and assesting the therapeutic effect and prognosis.

The Effect of Calcitonin Gene Related Peptide on Arrhythmia Caused by Acontine in Heterotopic Heart Transplant Rats

Wang Rong;Li Shuang;Ye Cuifei;Sheng Shuli;Yue Xiaohui

1996, 17(4):  277-279. 

Asbtract ( 633 )   PDF (2419KB) ( 202 )  

Related Articles | Metrics

Calcitonin gene related peptide(CGRP) is a most potent vasodilator.It plays an important role on arrhythmia.The purpose of this study was to observe the effects of CGRP on arrhythmia caused by aconitine in heterotopic heart transplant rats.The results showed : ①Both aceptor heart and transplanted heart had very weak reaction to CGRP.This suggested that the transplant heart has less CGRP receptors than normal heart.②CGRP can induce the appearance of P wave of electrocardiogram in one transplanted heart,this involved that CGRP may have a stimulation to sinus.

Detection of Chlamydia Trachomatis DNA in Prostatic Secretion of Chronic Prostatitis Using PCR

Zhang Yueqing;Zhou Mo;Wang Hong;Shao Qiang;Zhang Yuhai

1996, 17(4):  280-282. 

Asbtract ( 586 )   PDF (494KB) ( 190 )  

Related Articles | Metrics

The repetitive DNA sequences of Chlamydia trachomatis in prostatic secretion of chronic prostatitis was detected by using polymerase chain reaction (PCR).In 134 patients, 39 cases showed positive reaction (29.1%).All of 15 patients with other urogenital diseases but no significant inflammation ,which were selected to be the control,gave negative results.Because of high sensitivity and specificity,the PCR technique can replace the culture techinique,and becomes a major method for the diagnosis of Chlamydia.

论著

Treatment of 158 Cases Hepatocholangiolithiasis Using the Choledochofiberscope

Li Xiaochun

1996, 17(4):  283-285. 

Asbtract ( 504 )   PDF (2265KB) ( 272 )  

Related Articles | Metrics

One hundred and fifty-eight patients with hepatocholangiolithiasis were observed and treated by flexible choledochoscope since 1986.Among them, 96 cases were carried out with this method during operations.61 cases were relieved by flexible choledochoscope.Among 62 cases with hepatobiliary residue stones, 59 cases were taken out all stones with choledochofiberscope after operations.Among all cases, 5 cases failed, the success rate is 96.67%.The author suggests that the choledochofiberscope may play an important role in treatment of hepatocholangiolithiasis .

The Study of hCG and hPL in Uterine Myoma, Endometrium, Decidua and Chorion

Liu Fenzhe;Zhu Dingshou;Xu Qing;Liang Yuanjing;Shi Xiaolin

1996, 17(4):  286-289. 

Asbtract ( 663 )   PDF (514KB) ( 500 )  

Related Articles | Metrics

The immunohistochemical method was used to show the human chorionic gonadotropin (hCG)and human placental lactogen(hPL) positive cells in uterine myoma,endometrium,decidua and chorion of early human pregnancy,and to study the differentiation and clinical application of these cells.There were four kinds of hCG and hPL positive cells in decidua of early human pregnancy.Because it is difficult to identify them by HE staining,this study will provide a new way in the diagnosis of exrtauterine pregnancy and early uterine pregnancy.Furthermore,there is another kind of cells which is positive to hPL and negtive to hCG.It is proposed that these cells come from the cytotrophoblastic cells after moving into the endometrium.They may be of great value in the study of the chorionepithelioma.