Table of Content

15 July 1999, Volume 20 Issue 3

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论著

Photosensitive Anticancer Studies on Chinese Medicine Cortex Phellodendri

Liao Jing;E Zheng;Ning Tao;Nie Yuxiu;Chen Yulan

1999, 20(3):  153-155. 

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BGC823 human stomach cancer cells were cultured for the identification of photodynamic effects of Cortex phellodendri with illumination of 480 nm and 650 nm wave length. In the test groups with illumination, the herb extract diminished cell growth curve and the metabolism of 3-(4,5-dimethylthiazo1-2-yl)-2,5-diphenyl tetrazolium bromide(MTT). Futhermore, the significant decrease of the acid phosphatase (P<0.01) and the incorporation of ³H-TdR into cells (P<0.01) were demonstrated in the test groups.The progression of cancer cell cycle was postponed in S-phase(P<0.01) by the herb of 100 mL/L,but no chromosome adhering aberration appeared. The ultrastructural appearances showed that in the test groups, the mitochondria and the rough endoplasmic reticula of the cells became swollen and vesiculate and the ribosomes decreased.The result showed that Chineses medicine Cortex phellodendri has photosensitive effects on the cancer cells.

Effect of Newcastle Disease Virus Losota 4 on BT325 Multicellular Spheroids

Li Aihua;Pan Lizhen;Ma Hongru

1999, 20(3):  156-158. 

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The cytotoxic effects of NDV vaccine Losota-4 on multicellular spheroids model of human glioblastoma multiform BT325 were investigated. The results indicated that the diameter of spheroid decreased in all NDV titer (P<0.01). Survival assay showed that NDV could effectively kill glioblastoma cell (P<0.01).

Studies on Gastroelectric Activity and Gastric Motility in Wistar Rats by Computer

Li Mengyan;Liu Xuezong;Meng Yang;Qu Ruiyao;Jin Zongping;Hou Ancun;Zhang Shutian;Yu Zhonglin

1999, 20(3):  159-161. 

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The cutaneous electrodes, gastric electrodes and strain gauges were used to record the cutaneous gastroelectric activity, gastroelectric smooth muscle slow wave and gastric circle muscle motility in Wistar rats of the control group and the acute stress group through computer, respectively. The data were delt with by time-frequency analysis, and statistics analysis. The differences of the two groups suggested that: ①Cutaneous electrogastrography could reflect the gastric activities, reveal gastric physiological and pathological laws, be applied in clinic as an objective standard to diagnose gastric diseases and evaluate the therapeutic effect to a certain degree. ② Cutaneous electrogastrography reflected not only the electric activities of gastric smooth muscle, but also some information about gastric circle muscle motility.

The Property and Contributions of the Structural Elements of the External Skeletal Fixator

Zhang Tao;Yu Peixin

1999, 20(3):  162-167. 

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Based on the property of the structural elements of the external skeletal fixator (ESF), the contribution of individual structural elements was studied. 16 cases of complications related to the structure failure of ESF was analyzed. The correct principle and method in use of ESF were provided. The constraint to the bone segment degrees of freedom (DOF) and the optimization to stiffness of the ESF system were emphasized.

Study on the Immunity of Culture Cordyceps Sinensis Nutritional Liquid

Kong Xianghuan;Xiao Zhongxin;Wang Hong;Chu Jinhua;Yin Xuejun

1999, 20(3):  168-169. 

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In order to learn the immune modulating function of the culture Cordyceps sinensis nutritional liquid,mice were fed with the culture Cordyceps sinensis nutritional liquid. Specific humoral immunity and cellular immunity functional indexes were selected to study.After the mice were fed with the culture Cordyceps sinensis nutritional liquid for 20 days,the splenic IgMPFC significantly increased as well as the titre of serume antibody and the percentage of T-lymphocyte in medium and low dose groups. All differences of delayed hypersensitivity between the treated the group and control group were no significance. The experiment suggested that the culture Cordyceps sinensis nutritional liquid had great effect on increasing mice′s humoral immunity and the positive percentage of T-lymphocyte.

Influence of Stem Cell Factor Combined with Interleukin-3 on Expansion in Vitro of Murine Bone Marrow Cells and its Hematopoietic Reconstitution Capacity

Wan Suigui;Liu Congyan;Lu Jilian;Sun Xuejing;Shao Wenshuo;Tian Ding

1999, 20(3):  170-173. 

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Investigation was carried out on the effects of stem cell factor (SCF) in combination with interleukin-3(IL-3) on expansion of mice 5FU treated 3 days′ bone marrow cells(d3-5FU-BMC )in vitro using the method of colony culture and hematopoietic recovery in lethally irradiated mice transplanted with the expanded cells .The result demonstrated that the number of granulocyte-macrophage colony forming units(GM-CFU) and high proliferative potential colony forming units(HHP-CFU) increased (24.2±6.1) fold and (1.5±0.3) fold(P<0.05) respectively compared with the control group when SCF was used alone.When SCF combined with IL-3,the number of GM-CFU and HHP-CFU was (30.5±9.5) (P＞0.05 compared with SCF alone)and (13.6±4.5) times of that of the control group(P<0.05 compared with SCF alone). The result of mice bone marrow cells transplantation experiment demonstrated that the number of recipients’ peripheral blood cells which received lethally irradiation transplanted with expanded d3-5FU-BMC with SCF,SCF+IL-3 recovered earlier by 1-5 days than the control group.Not only the number of the peripheral blood cells of SCF+IL-3 group recovered the earliest,but also the number of transplanted cells were reduced more than 10 folds. The result suggested that SCF had an effect on expansion of mice bone marrow cells in vitro.There was synergistic effect between SCF and IL-3.The expanded bone marrow cells could accelerate early hematopoietic reconstitution and reduce the number of transplanted cells.

Relationships between Angiotensin Ⅰ Converting Enzyme Gene Polymorphism and Diabetic Nephropathy in Chiness Type Ⅱ Diabetes Mellitus

Wang Jianjun;Pan Ying;Sun Lin;Yang Ling;Li Hongbing;Fu Hanqing

1999, 20(3):  174-177. 

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Angiotensin Ⅰ converting enzyme (ACE) genotypes of 274 cases with type Ⅱ diabetes mellitus without nephropathy (NIDDM), 102 with diabetic nephropathy complicated with renal insufficiency (RI) and 124 diabetic nephropathy without renal insufficiency (NRI), and 242 controls were determined by polymerase chain reaction (PCR) amplification of the insertion/deletion polymorphism of ACE gene. Serum ACE levels were measured with ultraviolet photometry as well. The increased frequencies of D allele (0.52) and DD genotype (0.30) were observed in 102 subjects with RI compared with 242 control subjects (0.38 and 0.16 respectively, P<0.05 and P<0.001). The odds ratio associated with DD/II genotype was 2.91 (95% CI 1.52～5.58, P<0.01) for RI. Individuals with DD genotype had the highest serum ACE in all groups, and with II genotype the lowest levels. Patients with diabetic nephropathy and RI had higher serum ACE levels compared with controls (P<0.001). There was no difference in allele distribution between 274 NIDDM patients, 124 patients with NRI and the controls. ACE gene polymorphism is associated with serum ACE levels (r=0.65, P<0.01). This result suggested that the raised serum ACE concentration may play a role in the initiation and development of diabetic nephropathy; deletion polymorphism of the ACE gene might be an important risk factor of progression to end stage renal failure in Chinese type Ⅱ diabetes patients.

A Preliminary Study on RBC Immune Function in Rats with Experimental Membranous Glomerulonephritis

Wu Baoyu;Yuan Shenyuan;Wei Min;Li Boguang

1999, 20(3):  178-180. 

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The RBC C₃b receptor rosette (RBC C₃bRR) and RBC immune complex rosette(RBC ICR) in rats with experimental membranous glomerulonephritis were studied. It was found that there was significant reduction of activity of the RBC C₃b receptor in rats with membranous glomerulonephritis as compared with the control group. The results showed that the disturbance of RBC immune function might be related to the pathogenesis of membranous glomerulonephritis.

Observational Studies on Q-T_d^p by Body Surface Potential Mapping in 80 Normal Individuals

Yang Shuling;Xu Juntang;Xiao Jie;Li Junhua

1999, 20(3):  181-183. 

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To evaluate the feasibility of Q-T_d measurements under circumstances of body surface potential mapping(BSPM), QT_d^p(dispersion of interval of Q-wave onset to T-wave peak) was observed by using a computerized automatic mapping system in 80 normal individuals. The findings were compared with the 12-lead Q-T_d(the dispersion of Q-T interval). It was shown that: ① Q-T^p, Q-T_d^p, Q-T_c1d^p(corrected by Bazzett formula) and Q-T_c2d^p(corrected by Fridercia formula) were (292.69±37.75) ms, (36.77±7.40) ms, (40.23±9.04) ms and (40.11±7.73) ms respectively. ② Q-T_d and Q-T_c measured by BSPM and 12-lead ECG correlated well each other. ③ The differences of above parameters between three age groups(18～35 years, 36～60 years and 61～70 years) were not significant. ④ Q-T^p of female was longer than that of male(P<0.05), no significant difference was found in other parameters. In conclusion, Q-T_d^p measurement by BSPM might be more accurate and reliable than 12-lead ECG. The normal value of Q-T_d^p was less than 52 ms in this study.

Clinical Analysis of Heparin Coated Stenting for 28 Patients with Coronary Artery Disease

Wu Qiming;Liu Xuanzhong;Xi Yong'an;Song Ke;Zhang Longyou;Hu Hongyu;Song Yuqing

1999, 20(3):  184-185. 

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To evaluate the effectiveness of heparin coated stents on acute/subacute stent thrombosis and late in stent restenosis,the stents were implanted in 32 target lesions of 28 patients with coronary artery disease. The procedural success rate was 100% without death. No acute/subacute stent thrombosis were observed after stenting. 5 patients suffered from recurrent angina during the 6～24 months follow up, 3(17.9%) had in stent restenosis confirmed by coronay angiography. The result showed that heparin coated stent was effective in preventing acute/subacute stent thrombosis, but it could not reduce late in stent resenosis rates.

Bone Metabolism Change in Graves Disease

Ren Jiping;Wang Xue;Tian Jian;Lin Yuzhi;Yu Mei

1999, 20(3):  186-188. 

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In order to learn the changes of bone metabolism in Graves disease early, urinary deoxypyridinoline(Dpd), serum bone GLA protein(BGP), alkaline phosphatase(AKP), serum calcium, phosphorus and bone mineral density of lumbar_2～4, upper femur of 63 patients with Graves disease were tested. The result showed serum BGP and urinary Dpd were higher than the controls (P<0.05). BMD of lumbar 2～4 and the upper femur were lower than the controls (P<0.05) except those female patients younger than forty years old and those of WARD in female patients older than forty years old. The correlation analysis showed that serum BGP and urinary Dpd were positive correlated with FT₄, but not with duration of illness, serum BGP was negative correlated with femur (NECK, WARD′s triangle) (P<0.05). The results suggested that the test of BMD, urinary Dpd and serum BGP could detect the abnormal changes of bone metabolism in Graves disease, these tests were more specific and sensitive than those traditional methods.

Studies on Pulmonary Function in Patients with Non-insulin dependent Diabetes Mellitus

Gu Haitong;Tan Jie;Wu Baoyu

1999, 20(3):  189-191. 

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The pulmonary function in 84 patients with non-insulin-dependent diabetes mellitus was detected by pulmonary function meter.The results showed that parameters relating to ventilation such as FVC and VC and the parameter reflecting pulmonary diffuse function DLCO was much lower in diabetes group than those in the control group and was associated with duration, the amount of complications, and persistent higher glucose.

Clinical Analysis of the Relative Factors of Old Patients with Brain-heart Syndrome

Qi Baojun;Wang Suqiu

1999, 20(3):  192-194. 

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To analyze the relationship between acute cerebrovascular diseases with secondary coronary heart diseases, 116 cases of brain-heart syndrome (BHS) and 119 cases of non-brain-heart syndrome (NBHS) were analyzed contrastively. The result showed that BHS was found more commonly in the old men. About 68.6% of patients were with an average age older than 70 years old. The occurrence of BHS were associated with stroke type, lesion location and the severity of disease . In these BHS patients, brain hemorrhage (67.37%) was found more commonly than brain embolism (25.86%). Intraventricular hemorrhage or brainstem press always indicated a high BHS occurrence accompanied with a bad prognosis and high mortality. The main cause of death is pump failure. Some key points for treatment are earlier diagnosis, release from high ICP, ECG monitoring, prevention from pump failure and timely adjustment of arrhythmia.

The Association between Cerebral Infarction and Disorders of Speech

Xie Shuping;Liang Baohua;Guo Dongmei

1999, 20(3):  195-197. 

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Three hundred and fifty cases with acute cerebral infarction were included in this study for the analysis of language disturbances. According to the examination of Chinese aphasia(draft) of acute cerebral infarction (language disturbances). The series comprised 100 cases with dysarthria caused by bulbar palsy resulted from multiple infarction in cerebrum,infarction in pons or medulla oblongata,40 cases with cerebellar language caused by infarction in brain stem or cerebellum and 210 cases with various types of aphasia. The last group was sorted into peri-lateral fissure aphasia (n=71, 33.8%), watershed aphasia(60,28.6%), complete aphasia(31,14.8%),anomic aphasia(8,3.8%) and subcortical aphasia(40,19.0%). CT and MRI showed that the intracranial lesions corresponded to the types of aphasia in 195 cases(92.9%). The results showed that disorders of speech and language were related to the position of cerebral infarction and the lesion of CT(MRI).

The Effect of Nimodipine on Prevention and Treatment of Arrhythmia Caused by Experimental Encephalorrhagia

Liu Jin;Du Fenghe;Zhang Tong;Li Mingyue

1999, 20(3):  198-199. 

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To study the effect of nimodipine on the prevention and treatment of arrhythmia and brain water content, dynamic electrocardiogram and models of intracerebral hemorrhage were used in experimental rat.In the control group, encephalorrhagia and the treated group (encephalorrhagica+nimodi pine), premature ventricular beat were (3±5) thousands/d, (14±16) thousands/d, (7±7) thousands/d, respectively. There was no difference between the treated group and control group (P>0.05),both the control and treated group strikingly differed from encephalorrhagia group(P>0.05, P<0.01). The brain water content of encephalorrhagica group and the treated group was (81.04±0.46)% and respectively(80.60±0.58)%. There was remarkalbe difference between them (P<0.05).

Clinical Experience of Fractures of Extremities Treated with External Fixators

Jiang Yuguan;Cui Fengqi

1999, 20(3):  200-201. 

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One hundred and sixteen cases with fractures of extremities were treated with external fixation using modular external fixators . The rate of open fracture is 55%. The duration of follow up was from 3 months to 5 years. The rate of fracture healing was 93%. Modular external fixators provided good fixation for treating long bone shaft fractures of extremities, especially open or infected fractures with severe soft tissues injuries. With the advantages of secure fixation, high rate of fracture healing, allowing early mobilization at the joints around fracture site, being easy to operate and low cost, modular external fixators were especially suitable for county hospitals where the instruments and surgical technique were relatively undeveloped.

The Significance of the MDR₁ Gene Products GP₁₇₀ Expression in the Bladder Cancer Tissue

Zhang Zishun;Zhao Yuqian;Wu Changli

1999, 20(3):  202-204. 

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The drug resistance of the tumor cells is the important factor in the failure of chemotherapy for cancer. To demonstrate the relation between the MDR₁ products and the failure of chemotherapy for bladder cancer, the 72 cases of bladder cancer were studied with the immunohistochemical method. The restults suggested that the positive rate and the strong positive rate of the MDR₁ products in the cases, in which the chemotherapy with mitomycin pouring into the bladdler post-operation was fail, were higher than those in the initial stage of bladder cancer. The positive and strong positive expressions of MDR₁ products in the high differentiation tumors were higher than those in the low differentiation tumors. This research indicated that the outcome of the chemotherapy for bladder cancer is relevant to the amounts of the MDR₁ products in the bladder cancer tissue.

The Clinical Analysis of 202 Cases of Oligohydramnios

Liu Yuhuan;Wang Ying

1999, 20(3):  205-207. 

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To investigate perinatal diagnosis of oligohydramnios and outcome of perinatal fetus methods 202 cases of oligohydramnios were reviewed. The incidence of oligohydramnios, from January, 1988 to July, 1998, was 1.47%(202/13 738). The prolonged pregnancy was 52.48 % (106/202). IUGR, EPH syndrome and prolonged pregnancy were common complications. The accordance rate that ultrasonography diagnosed oligohydramnios was 72.77%(147/202). The incidence of fetal distress and asphyxia neonatorum were 44.55%(90/202) and 2.48%(5/202) respectively. The death rate of perinatal fetus was 0.99%(2/202). The cesarean section rate was 92.08%(186/202). The result showed that ultrasonography is a simple and effective method diagnose oligohydramnios in the period of gestation. Early diagnosis and quickly performed ceasarean section is the key to improve the outcome of perinatal fetus of oligohydramnios.

Influence of Overweight and Obesity on Lipids and Blood Pressure

Gao Guojing

1999, 20(3):  208-210. 

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High, body weight, blood pressure, lipids and blood glucose of 459 subjects working in capital university of medical sciences were examined. The results showed: With increasing age, total cholesterol and triglyceride increased; with increased body mass index, the level of lipids and blood pressure increased, and morbidity of high lipids, lipoprotein and essential hypertension increased. Stepwise multivariate analysis diclosed that age alone and jointly positively correlated with total choleserol and triglyceride in women; body mass index alone and jointly positively correlated with triglyceride. It was suggested that control body weight was very important in middle aged and elderly subjects.