Table of Content

15 October 1995, Volume 16 Issue 4

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论著

Effects of Acute Repeated Hypoxia on Hypoxia Tolerance of BALB/C Mice

Li Ling;Lu Guowei;An Yangyuan

1995, 16(4):  249-252. 

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With repeated exposure of BALB/C mice to hypobaric hypoxia,the tolerance time of the mince in the first run of exposure was 17.3 min in average and those of the subsequent 2nd,3rd and 4th runs were 3,6 and 7 times longer than that of lst run respectively,the animals'survival times in hypobaric chamber and after cyanide intoxication were 26 and 2.6 times longer respctively than that of the control without exposure to hypoxia.These results indicute that the BALB/C mices tolerance to hypoxia is significantly increased through acute and repetitive exposure to hypoxia.

Ornithine Decarboxylase Inhibitor Reduces Brain Damage of Focal Ischemia in Cats

Hu Ying'an;Hou Minglun;Yang Haipuig

1995, 16(4):  253-255. 

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Ornithine decarboxylase(ODC), the rate limiting enzyme of polyamine metabolism,is felt to be important in the generation of ischemic brain edema and infarction.We measured Evens blue staining,regional blood flow(rCBF) and specific gravity of brain tissue following focal ischemia in cat middle cerebral artery occlusion model.Pretretment with ODC inhibitor α-difluoromethylornithine(DFMO) decresed Evens blue staining from 29.6% to 17.2% (P<0.01) and also reduced specific gravity from 1.028 to 1.016 (P<0.01).The rCBF increased by a maximum of 394% as compared with control group.These data suggest that polyamine production and ODC activity play a significant role in the development of ischemic edenia.

Toxicological Study of Cordyceps Sinensis Cultivated by Artificial Fermentation on Its Safety Evaluation Ⅱ. Mutagenicity Study

Kong Xianghuan;Jiang Baoji;Wang Huiqin;Yin Xuejun;Tang Yuping;Ma Zhongjie;Shen Jiaqin;Zhang Hongwei;Xiao Zhongxin

1995, 16(4):  256-258. 

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Study on mutagenic effects of artificially fermented Cordyceps sinensis(AFCS) was carried out by three mutagenicity tests, namely,bone marrow cell micronucleus assay,spermatocyte aberration analysis in mice and Ames test.There were no significant difference betweenthe frequencies of micronuclei in three dosed groups and that in controls.Similarly,spermatocyte aberration analysis also showed negative result.In Ames test,the sample did not show any direct or indirect-acting mutagenicity to the test strains.The results demonstrate that AFCS does not have mutagenic effects on both somatic and reproductive cells of mice,and also,on salmonella strains.

A Morphologic Study on the Bilaterally Autotransplanted Ovary in White Mouse

Liang Yuanjing;Shi Xiaolin;Zhu Dingshou

1995, 16(4):  259-262. 

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Two ovaries of the female mouse aged 30 postnatal days were autotransplanted subcutaneously in order to study the growth and secretion of the transplanted ovaries.The ovarian graft was surrounded by a thick layer of connective tissue.Follicle necrosis began on day 2.Blood cells infiltrated into the necrotic area.Four days after transplantation ,there were some primodial follicles and preantral follicles in the outer cortex of the ovary.On day 7,a few antral follicles, graffian follicles and interstitial-land-like tissue could be detected.On day 14 to 21, there were luteinic tissue in the graft.Necrosis disappeared.28 to 72 days after grafting, a lot of follicles in different stages of development, corpora luteas and interstitial glands could be found.The estrus cycle emerged 7.9±1.7 days after transplantation.This study indicates that bilaterally autotransplanted ovaries can grow and secrete sex hormones which maintain the estrus cycle.

论著

Separation and Determination of Eicosapentaenoic Acid and Docosahexaenoic Acid in Fish Oil by High Performance Liquid Chromatography

Guo Aimin;Wang Hui;Yin Na;Zhang Shuhua;Yan Fusheng

1995, 16(4):  263-267. 

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The method of high performance liquid chromatography analysis for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the anchovy oil was reported.The sample was determined with HPLC system of model Waters and μ-Bondpark C₁₈ colume (30cm×4 mm i.d).The EPAand DHA in fish oil was saponified with 0.5 mol/L KOH-C₂H₅OH,esterified with triethylamine-ω-Bromoacetopnenone, and inject directly into the system of HPLC.UVdetection was performed at 254 nm,with the mobile phase of Methanol-water(88:12).The contents of the EPA and DHA in the sample were determined by standard curve.The recovery rates of EPA and DHA were 93.0%～98.0% and 93.0%～100.0% respectively.The relative standard deviations of EPA and DHA were 0.41%～0.86% and 0.67%～1.20% (n=7).This method is simpie,rapid and with good repeatability.

Isoelectric Focusing of Total Cell Proteins of Four Giardia lamblia Isolated from China and America

Liu Hua;Lu Siqi;Wang Fengyun;Wang Zesheng

1995, 16(4):  268-270. 

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The total cell proteins obtained from the trophozoites of four Giardia lamblia isolates, of which three were isolated from China (C₁, C₂, and C₃ ), and one from America(CDC), were studied by using an isoelectric focusing technique.It was shown that each of the three domestic isolates produced identical banding patterns,of which bands rang from pl 3.6 to 8.3.The banding pattern of the Anierican isolate was different from those of the Chinese isolates,which produced two adclitional new bands.The two distuict bands located at pI 7.1 and 7.8 respectively.The present study shows that geographical separation may be one of the reasons which causes difference between Giardia isolates from different geographical regions.

The Role of Electron Microscopy in the Differential Diagnosis of Lymphoma

Sun Yongping;Tian Mi;Ji Xiaolong

1995, 16(4):  271-273. 

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Forty-seven tissue samples which were difficult to diagnose by light microscopy(LM) were examined by electron microscopy(EM) from 1984 to 1990 in the PLA's General Hospital,aiming at comparing the diagnostic results of CM and EM.The differential diagnosis of non-differentiated small cell tumors was very hard in route pathological-examination, but they all had the charateristics of their own,being helpful for differential diagnosis.However,there was no any detail report for the differential diagnosis of lymphomas by EMin China.We analyzed the results of lymphomas.Of the 47 cases of lymphomas examined by EM,there were 32 cases of non-Hodgkins lymphoma, 2 cases of histiocytic hyperplasia and lymphocytic leukemia each, and 1 case of malignant histiocytic hyperplasia, lymphomatous granuloma and histocytic reactive hyperplasia of bone marrow each.These results show that EM is helpful for the differential diagnosis of small cell tumors.

Study of Immunoglobulin Gene Rearrangement in Patients with Multiple Myeloma by PCR Techniques

Chen Wenming;Zhu Jiazhi;Zhang Peng;Sun Weihong;Li Shulan;Li Guiying

1995, 16(4):  274-277. 

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In this study, immunoglobulin heavy chain (IgH) gene rearrangement in peripheral blood or bone marrow smear of multiple myeloma (MM) patients by PCR techniques was analyzed.Out of 21 patients, 17 were found to be positive and 4 negative, two of which were light chain type.This positive rate of peripheral blood IgH gene rearrangement was the same as that of bone marrow.These results suggest that the detection of IgH gene rearrangement is one of the most sensitive method for the diagnosis of MM,which can counteract the weaknesses of morphology and serology.

论著

Influences of Preload Afterload and Contractility of Myocardium on Left Ventricular Diastolic Function

Hua Qi;Shi Shuying;Wang Jiarui

1995, 16(4):  278-281. 

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To investigate the influences of preload,afterload and contractility of myocardium on left ventricular diastolic function,134 patients suffering from different kinds of diseases were examined by echocardiography.The patients were divided into five subgroups according to diameter of left atrium,end diastolic volume of left ventricle,mean arterial blood pressure, mean velocity of circumferential fiber shortening and ejection fraction of left ventricle.It was found that:①Variation of preload influenced chiefly early diastolic filling parameters,peak flow velocity of early diastole(EPFV)and time-velocity integral of early diastole (ETVI) which increased with increasing preload.②Variation of afterload influenced not only early, but also late diastolic filling parameters.ETVI increased,peak flow velocity of atrial contraction(APFV) and time-velocity integral of atrial contraction(ATVI) decreased,and the ratio between APFV and EPFV and between ATVI and total time-velocity integral (TTVI) re-duced with decreasing afterload.③Decreased contractility of myocardium as manifested by a reduction of EPFV and ETVI,and a significant componsatory increase in APFV,ATVI and the ratio between APFV and EPFV and between ATVI and TTVI in order to maintain the cardiac output.

Evaluation of 246 Cases with Permanent Pacemaker through Different Veins

Na Kaixian;Liu Qingshan;Shen Luhua;Gu Fusheng

1995, 16(4):  282-283. 

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246 cases with permanent pacemaker through different veins were reported which consisted of 135 cases of men and 111 cases of women.In 122 cases cephalic vein was used with a successful rate of 82% (100 cases), dislocation of 7.4% (9 cases) and local infection of 2.5/00 (3 cases).In 12 cases external jugular vein was used with a successful rate of 91.7%(11 cases ),damage of pacing catheter and local skin infection in 2 cases.In 112 cases subclavian vein was used with a successful rate of 97.3% (109 cases), local infection ,pneumothorax and dislocation in 1 case each.

Measurement of Plasma Parathyroid Hormone and Relation to Plasma Renin Activity in 30 Patients with Essential Hypertension

Xu Xiuying;Zhang Jianfeng;Feng Weijie

1995, 16(4):  284-286. 

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Plasma parathyroid hormone (PTH) level has been measured by radioimmunoassay(RIA) in 18 persons with normal blood pressure and in 30 patients with essential hypertension.The result suggested that PTHlevel was elevated in hypertensives[(86.5±40.2) ng/L] compared with normal tensive controls [(59.4±15.6) ng/L ,P<0.01].Continous relationship between PTH level and PRA in hypertensives, r=-0.385,P<0.05.

A Study on Links of Stress Ulcer and the Serum Levels of Gastrin Glucagon and Somatostatin in Rats

Liang Peixia;Yang Zhaoxu;Zhang Shaodong;Wu Jianzhong

1995, 16(4):  287-289. 

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This article deals with the links between three gastrointestino-hormones and irritable ulcer in“cold-ties”induced ulcer model.Our experiment indicated that: ①The serum gastrin levels of the stress group were significantly higher than that of the control.This might be due o the excitement of vagus nerve under stress.② The serum levels of somatostatin in stress group were lower than that of the control ,this indicated that the inhibihition of somatostatin in gastro-secretin was reduced which in turn caused hyperhydrochloria.③The serumglucagon correlated positively with serum gastrin level and negatively with blood sugar.These peptides might function as gastrointestinal hormones and neurotransmitters in the CNSand peripheral nervous system.

Optimum Choice of the Low Frequency Filtering Function in ²⁰¹Tl Myocardial Tomography

Wang Tie;Zhang Jingu

1995, 16(4):  290-292. 

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Choice of the filtering function in single photon emission computerized tomography (SPECT) is of great clinical importance and will significantly affect the quality of the tomographic image.11 normal subjects and 22 patients with coronary arterial disease were studied using ²⁰¹Tl SPECT imaging.Hamming Hann filter was compared with Wiener filter in the reconstruction of SPECT imaging.The purpose of this study was to select an optimum filter for ²⁰¹Tl myocardial tomography.

Clinical Observation of Therapeutic Effects on Hepatitis B Virus by Cinobufacini

Gao Qinglin;Li Yuying;Guo Jianqiang;Li Yubin

1995, 16(4):  293-295. 

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33 cases of chronic hepatitis B (32 cases for control) were treated by cinobufacini.The same management was used for both control and treating groups ,except an additional treatment by injecting 4 mL of cinobufacini per day intramuscularly,for the treating group.There were 45 days in one course of treatment.Afrer one course,the negative conversion rates of HBeAg and HBV-DNA in the treating group were 44.84% and 34.61% respectively, while in the control group they were only 7.14% and 0 respectively.This observation suggests that short-term effect of cinobufacini is rather satisfactory for treating the chronic hepatitis B.